Genomic evidence that simian virus 2 and six other simian picornaviruses represent a new genus in Picornaviridae

Molecular phylogeny and proposed classification of the simian picornaviruses.

The simian picornaviruses were isolated from various primate tissues during the development of general tissue culture methods in the 1950s to 1970s or from specimens derived from primates used in biomedical research. Twenty simian picornavirus serotypes are recognized, and all are presently classified within the Enterovirus genus. To determine the phylogenetic relationships among all of the sim...

A Functional Simian Virus

We used two recombinant plasmids, one containing wild-type simian virus 40 DNA (pSVR1) and the other containing a simian virus 40 genome with a defective origin of replication (pSVR1-origin-minus) to transfect NIH3T3 cells. Quantitation of T-antigen synthesis by indirect immunofluorescence at 48 h after transfection with either DNA revealed the same percentage of T-positive nuclei. The transfor...

Control of simian virus 40 gene expression in adenovirus-simian virus 40 hybrid viruses. Synthesis of hybrid adenovirus 2-simian virus 40 RNA molecules in cells infected with a nondefective adenovirus 2-simian virus 40 hybrid virus.

The effect of interferon on simian virus 40 (SV40) and adenovirus 2 (Ad2) T antigen synthesis has been examined in cells infected with SV40, with Ad2, and with a nondefective Ad2-SV40 hybrid virus, Ad2(+)ND(4). The induction of SV40 T antigen by SV40 was highly sensitive to interferon, whereas the induction of Ad2 T-antigen by Ad2 was resistant. This difference in interferon sensitivity was als...

Virus-Induced IL-2 Expression by Central Virus/Simian Human Immunodeficiency Dysfunction of Simian Immunodeficiency

Restriction endonucleases, simian virus 40, and the new genetics.

Introduction Some 3.5 years ago the study of heredity took a chemical turn when Avery and his colleagues discovered that deoxyribonucleic acid-DNA-is the " transforming principle " that converts bacteria from one genotype to another. Watson and Crick's structural model of DNA then provided the basis for investigating its role as the hereditary material. There followed rapid advances in the bioc...